Nanopore Sequencing @ CRS

Platform

Nanopore sequencing enables real-time, long-read analysis of DNA or RNA molecules, providing insights into sequence composition, structural variation, and epigenetic modifications. Unlike short-read sequencing methods, nanopore technology reads single molecules as they pass through a nanoscale pore, allowing the capture of very long fragments and complete genomic or transcriptomic structures. This approach is particularly useful for detecting large structural variants, resolving repetitive regions, or sequencing full-length microbial genomes from plasma or other sample types.

Potential Applications

• Detection of structural variants and large genomic rearrangements
• Full-length microbial genome sequencing from plasma or environmental samples
• Epigenetic modification profiling, including DNA methylation
• Rapid pathogen identification and monitoring in infectious disease studies
• Transcriptome analysis of full-length RNA molecules
• Direct RNA sequencing for isoform identification, splicing analysis, and RNA modification detection