Workflow
Metagenomic sequencing enables comprehensive analysis of microbial DNA isolated from mixed samples, providing insights into the diversity, functional potential, and interactions of microbial communities within a sample. Based on shotgun whole genome sequencing, the informatics approach identifies microbial fragments present in a sample, allowing for unbiased detection and characterization beyond targeted assays. Whether you are investigating bloodstream infections, monitoring microbial dynamics, or exploring host–microbe interactions, we can take your extracted DNA and generate high-quality, multiplexed libraries ready for next-generation sequencing.
These workflows are based on experiences in the Kisat lab at CPM, who recently developed a laboratory and computational workflow for metagenomic sequencing of plasma DNA and enrichment of microbial DNA fragments (Dominguez et al. Clinical Chemistry 2025, https://academic.oup.com/clinchem/article/71/5/567/8069772 and Zhang et al. medRxiv 2026, https://www.medrxiv.org/content/10.1101/2025.11.06.25339688v1).
- Double-Stranded DNA Library Preparation
- Single-Stranded DNA Library Preparation
- Enrichment of microbial DNA by size selection (for plasma samples)
Potential Applications
- Microbiome analysis in plasma and other biofluid samples
- Pathogen detection for infectious disease
- Microbiome analysis in tissue samples
Metagenomics Contacts
Mehreen Kisat, MBBS, MS
Credentials: Department of Surgery
Position title: Assistant Professor
Email:
kisat
Address:
CRS Faculty Affiliate
Eddie Dominguez, Ph.D
Credentials: Kisat Lab
Position title: Scientist I
Email:
researchservice
Address:
CRS Scientific Lead
Haikun Zhang, Ph.D
Credentials: Murtaza Lab
Position title: Postdoctoral Scientist
Email:
researchservice
Address:
CRS Informatics Staff
Price/Sample
| Member | Non-Member | |
| Library prep, QC, P2 100c flow cell | $1,123 | $1,858 |
| Size Selection | $20 | $35 |
| DNA Isolation | $16 | $16 |
Price is dependent on the scope of work and flow cell used