Illumina Sequencing @ CRS

Platform

The NextSeq 2000 platform enables high-throughput, short-read analysis of DNA and RNA, providing accurate and comprehensive coverage for a wide range of genomic applications. The platform uses sequencing-by-synthesis chemistry to generate millions of reads in parallel, allowing detailed examination of genetic variants, transcriptomes, and epigenetic modifications. With flexible output options and rapid turnaround, the NextSeq 2000 supports both small- and large-scale studies while maintaining high data quality and reproducibility.

Potential Applications

  • Whole-genome and targeted DNA sequencing
  • RNA-Seq for transcriptome profiling and gene expression analysis
  • Metagenomic sequencing for microbial community characterization
  • ChIP-Seq, ATAC-Seq, and CUT&Tag library sequencing
  • Detection of genetic variants, including SNPs, indels, and structural variations

Illumina Sequencing

Eddie Dominguez

Eddie Dominguez, Ph.D

Credentials: Kisat Lab

Position title: Scientist I

Email: researchservice@precisionmedicine.wisc.edu

Address:
CRS Scientific Lead

Flow Cell Output

P1 P2 P3 P4
Cycles  (100, 300, 600)  (100, 200, 300, 600) (100, 200, 300)  (50, 100, 200, 300)
Single Reads 100M 400M 1.2B 1.8B
Paired-end Reads 200M 800M 2.4B 3.6B

Flow Cell Price

Member Non-member
P1 100c  $711 $1,316
P1 300c  $981 $1,586
P1 600c $1,492 $2,097
P2 100c  $1,072 $1,677
P2 200c $2,014 $2,619
P2 300c  $2,671 $3,276
P2 600c $2,907 $3,512
P3 100c $2,288 $2,893
P3 200c $3,168 $3,773
P3 300c $4,224 $4,829
P4 50c $1,766 $2,371
P4 100c  $2,649 $3,254
P4 200c $3,746 $4,351
P4 300c $4,768 $5,373

Price/Sample

Member Non-Member
Illumina library prep, QC, P1 100c flow cell $762 $1,497

Price is dependent on the scope of work and flow cell used