University of Wisconsin–Madison

Illumina Sequencing @ CRS

The word research on a red tile mosaic.
The word “research” adorns a red tile mosaic wall at the Discovery Building at University of Wisconsin–Madison on April 30, 2022. (Photo by Jeff Miller / UW–Madison)

Platform

The NextSeq 2000 platform enables high-throughput, short-read analysis of DNA and RNA, providing accurate and comprehensive coverage for a wide range of genomic applications. The platform uses sequencing-by-synthesis chemistry to generate millions of reads in parallel, allowing detailed examination of genetic variants, transcriptomes, and epigenetic modifications. With flexible output options and rapid turnaround, the NextSeq 2000 supports both small- and large-scale studies while maintaining high data quality and reproducibility.

Potential Applications

  • Whole-genome and targeted DNA sequencing
  • RNA-Seq for transcriptome profiling and gene expression analysis
  • Metagenomic sequencing for microbial community characterization
  • ChIP-Seq, ATAC-Seq, and CUT&Tag library sequencing
  • Detection of genetic variants, including SNPs, indels, and structural variations

Contacts

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Flow Cell Output

 P1P2P3P4
Cycles (100, 300, 600) (100, 200, 300, 600)(100, 200, 300) (50, 100, 200, 300)
     
Single Reads100M400M1.2B1.8B
Paired-end Reads200M800M2.4B3.6B

Flow Cell Price

 MemberNon-member
P1 100c $711$1,316
P1 300c $981$1,586
P1 600c$1,492$2,097
   
P2 100c $1,072$1,677
P2 200c$2,014$2,619
P2 300c $2,671$3,276
P2 600c$2,907$3,512
   
P3 100c$2,288$2,893
P3 200c$3,168$3,773
P3 300c$4,224$4,829
   
P4 50c$1,766$2,371
P4 100c $2,649$3,254
P4 200c$3,746$4,351
P4 300c$4,768$5,373

Price/Sample

 MemberNon-Member
Illumina library prep, QC$51$51

The price/sample may change for sample sizes greater than 32 and the scope of work.